NSW Public Health Bulletin archive

Using MLVA to type strains of Typhimurium in New South Wales

Gwendolyn L. Gilbert

New South Wales Public Health Bulletin 19(2) 29–31 http://dx.doi.org/10.1071/NB07116 Published online: 28 February 2008

  • Citation

  • PDF

About the author/s

Gwendolyn L. Gilbert

Abstract

Summary: Phage typing has been the traditional strain typing (or ?fingerprinting’) method used in Australia for surveillance of common salmonella serovars (such as Typhimurium) and outbreak investigations. The need for more accessible, discriminatory and objective methods has been recognised but, until now, none has been widely accepted. Recently, the molecular typing method, known as MLVA (multilocus variable number tandem repeat analysis), has been applied to several serovars and promises to provide faster strain typing and cluster identification than phage typing, with comparable or better sensitivity. The present article is intended as a short primer on MLVA typing, which has recently been introduced into routine use at the New South Wales Enteric Reference Laboratory at the Centre for Infectious Diseases and Microbiology, Institute of Clincial Pathology and Medical Research, Westmead.